Quantitative spectrofluorometric assay detecting nuclear condensation and fragmentation in intact cells

Abstract At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy flow cytometry. However, none of the methods used for quantitative spectrofluorometric assessment. Therefore, aim present study was to develop a assay detection intact cells. We human hepatoma HepG2 renal HK-2 cells cultured 96-well plates treated with potent apoptotic inducers (i.e. cisplatin, staurosporine, camptothecin) 6–48 h. Afterwards, were incubated 33258 (2 µg/mL) increase after binding dye DNA measured. The developed capable detect changes caused by all tested inducers. Then, we compared outcomes other detecting cell impairment apoptosis WST-1 glutathione tests, TUNEL, ladder, caspase activity, PARP-1 JNKs expressions). found that our provided results same sensitivity as TUNEL but advantages being fast processing, low-cost high throughput. Because can be typical markers death, especially apoptosis, suppose could become routinely method characterizing death processes.